We posted last week about Pestalotiopsis and the role it may play in generating the lesions on Red Mangrove leaves we’ve documented. Here are some photos that show part of the process involved in determining if Pestalotiopsis is the causal agent of the lesions. In short, leaves from Red Mangroves showing no symptoms of lesions were plucked from the seedlings and subsequently inoculated with conidia (asexual spores) of Pestalotiopsis. The inoculated leaves have been incubating for about 7 days so our first trial inoculation experiment is almost complete! We will post back with more updates as they become available.
Snapshots of an inoculation experiment
2 Comments
Comments are closed.
I was wondering whether such experiment is difficult to conduct. Significant differences in DON levels among the barley genotypes (P < 0.0001) were observed for only one point-inoculation experiment?
The basis of this experiment is Koch’s Postulates. I am not interested in mycotoxins such as DON for this experiment, so, my design is very simple and follows Koch’s Postulates. I am not an expert on mycotoxins, so I would consult the literature more.
Best,
Ryann